Clean, fresh lab coats and glove should be worn at all times in the hood area and removed as soon as the area is left.
UV light in hood should be on whenever hood is not in use. This means that all UV-sensitive materials (e.g., media) should be kept out of the hood and shadowed from UV.
Materials often brought into the hood (pipettors, pipettes, hemocytometer, etc.) should be kept in a clean cupboard when not in use.
At beginning of work, place all necessary equipment in hood and try to avoid removing arms repeatedly through the work period.
Do not leave bottles uncovered for more than is absolutely necessary.
Dry bottles that have been warming in water thoroughly and rinse generously with ethanol before introducing into the hood.
During liquid transfer, pipettes should be discarded if any possibility of contact with a potentially contaminated solution has occurred (even the lightest touch).
Last drops in pipettes should not be evacuated, since aerosols and splashes result.
While ethanol is the only practical disinfectant for washing gloves, flasks, etc., stronger compounds (e.g. quaternary ammonium disinfectants) can be used for washing down surfaces.
Doors to culture room should be kept closed at all times.
Filters in pipettors should be changed regularly.
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